About IVF Testing

After a detailed review of the patient’s history including old medical records, the Physician will determine all the necessary testing still required. Usually, tests that have been performed within the last year will not be repeated. Otherwise, the patient will be informed to contact the office with the start of a period to schedule the appointments to perform the Pre-Treatment cycle testing. This may include the following:

  • Blood test to check the ovarian reserve, called FSH and Estradiol, done on the 2nd or 3rd day of the period. This test can be done at a local laboratory also. A test requisition can be faxed at the patient’s request.
  • An ultrasound to check the Antral Follicle Count and ovarian reserve along with fluid instillation inside the uterine cavity to assess the adequacy of the uterus to carry a pregnancy (Fluid Ultrasound Examination).
  • During the same visit, the Physician will perform a Mock Embryo Transfer, which is a measurement of the uterine depth so we know exactly where to deposit the embryos, in addition to prepare ourselves in case of a difficult transfer. Cervical cultures for infectious diseases will also be completed at this time.

Completion of the Pre-Treatment testing is required by State and Federal law, in addition to in house Policy. A list of these tests is outlined in a later section. Some of these tests can also be performed at a local laboratory. A Semen Analysis is part of this testing. As mentioned above, we will not repeat any tests that were performed within a year of the projected treatment cycle with us.

What follows is the list of tests that we require. Some of these tests might not apply to a particular type of patient. Additionally, a series of different tests can be requested in certain conditions that are not on this list. The following gives a brief description of some of the required tests.

  • HIV-1 & 2: AIDS causing virus. Anonymous egg/sperm donors who test positive will not be able to donate. A recipient/surrogate who tests positive will not be able to carry a pregnancy.
  • HTLV-1 & 2 (Human T-Lymphocytic Virus): A virus similar to the HIV virus that can be associated with the causation of leukemia and certain neurologic diseases. The presence of HTLV will preclude any treatment involving the use of the individual as the egg source or the recipient. Anonymous egg/sperm donors who test positive will not be able to donate. A recipient who tests positive will not be able to carry a pregnancy.
  • Hepatitis B surface antigen (HepBsAg): A blood test to check for the presence of the Hepatitis B virus. In case it is positive, treatment can still be performed but a consent form must be signed. Anonymous egg/sperm donors who test positive will not be able to donate.
  • Hepatitis B core antigen (HepBcore Ab): A blood test to check for the presence of the Hepatitis B virus. In case it is positive, treatment can still be performed but a consent form must be signed. This test if often positive in patients who have acquired immunity to Hepatitis B, naturally or through immunization. Anonymous egg/sperm donors who test positive will not be able to donate.
  • Hepatitis C antibody (HepC Ab): A blood test to check for the presence of Hepatitis C virus. In case it is positive, treatment can still be performed but a consent form must be signed. Anonymous egg/sperm donors who test positive will not be able to donate.
  • VDRL/RPR (Syphilis): A blood test to check for the presence of Syphilis, a sexually transmitted disease. Anonymous egg/sperm donors who test positive will not be able to donate.
  • CMV-IgM and CMV IgG (Cytomegalovirus): A test for the Cytomegalovirus that can cause serious congenital fetal damage if a woman is pregnant with the active virus present.
  • Gonorrhea and Chlamydia cultures: This test is performed during a pelvic examination and involves checking for the presence of the sexually transmitted diseases of Gonorrhea and Chlamydia. If present, both partners will need to be treated prior to treatment. Anonymous egg/sperm donors who test positive will not be able to donate for 12 months.
  • FSH/Estradiol: Blood hormone tests done on the 2nd or 3rd day of a menstrual cycle to check the ability of the ovaries to respond to fertility drugs and help us decide the amount of medication needed. This test if often combined with the Antral Follicle Count.
  • TSH: Thyroid stimulating hormone. This test checks the function of the Thyroid gland which can play a significant role in the success of infertility treatment and the health of the fetus during pregnancy.
  • Prolactin: A hormone secreted by the pituitary gland that can interfere with ovarian stimulation and embryo implantation.
  • Rubella: This is a test to check for the immunization status for German Measles. If the patient is not immune to it, she will have to be immunized and the treatment postponed by 1-2 months.
  • Varicella: This is a test to check for the immunization status against Chickenpox. If the patient is not immune to it, she will have to be immunized and the treatment postponed by 1-2 months.
  • CBC: Complete Blood Count. This test checks for abnormalities in blood, such as anemia.
  • Blood type and Antibody Screen: It is important to know the blood type of the patient prior to treatment especially if the recipient is negative and the sperm source is positive.
  • Pap smear: Done to check for cervical precancerous lesions.
  • CF screening: Cystic fibrosis screening. This test is performed to check the carrier status of this common genetic disease which occurs frequently in the general population with an incidence of 1/22.
  • Urine drug/cotinine screen: Done for surrogates and egg donors only.
  • Pelvic ultrasound to assess the uterus: The purpose of the ultrasound examination is to check the integrity of the uterus and rule out the presence of polyps or fibroids that can interfere with the success of the treatment. In addition, it is done to evaluate the status of the ovaries and rule out the presence of any cysts.
  • Hemoglobin Electrophoresis: A test commonly done to rule out the presence of genetic blood diseases such as Sickle Cell Disease, Hemophilia or Thalassemia.
  • Immune testing: These can include multiple tests such as Antiphospholipid antibodies, Thyroid antibodies, ANA, Lupus Anticoagulant, DQ genotyping, NK Cell Activity measurement, Immunophenotyping etc.. The physician will determine who is eligible to perform any of these tests.
  • Genetic testing: These may include testing for rare genetic disorders such as Gaucher d., Canavan d., Prader-Willi, Tay Sachsa, etc. Genetic testing is performed depending on the history of the patient.
  • Semen Analysis: A test to check for the sperm count, motility and morphology.
  • SCSA: Sperm Chromatin Structure Assay. This test is to check for DNA fragmentation in the nucleus of sperm. An abnormally high level of fragmentation is associated with poor treatment outcome. We perform this test in men with history of repeated failures with no apparent cause.

In the following table, the most commonly required tests are shown for a particular type of patient:

Female Partner

Male Partner

 Egg Donor

 Sperm Donor

Surrogate

Surrogate’s Partner

HIV 1,2

HIV 1,2 HTLV 1,2

HIV 1,2

HIV 1,2 HTLV 1,2

HIV 1,2

HIV 1,2

Hep B s Ag

Hep B s Ag

Hep B s Ag Hep B core

Hep B s Ag Hep B core

Hep B s Ag

Hep B s Ag

Hep C Ab

Hep C Ab

Hep C Ab

Hep C Ab

Hep C Ab

Hep C Ab

Syphilis

Syphilis

Syphilis

Syphilis

Syphilis

Syphilis

Chlamydia Gonorrhea

Chlamydia Gonorrhea

Chlamydia Gonorrhea

Chlamydia Gonorrhea

CBC

CBC

CBC

CBC

CBC

Prolactin

Prolactin

Prolactin

TSH

TSH

TSH

Blood Type Ab Screen

Blood Type

Blood Type

Blood Type

Blood Type Ab Screen

Rubella

Rubella

Varicella

Varicella

CMV

CMV

CMV

Pap smear

Pap smear

Pap smear

Urine Drug Screen

Urine Drug Screen

Cystic Fibrosis (Optional)

Cystic Fibrosis (Optional)

Cystic Fibrosis

Cystic Fibrosis

Other Genetic Testing

Other Genetic Testing

Other Genetic Testing

Other Genetic Testing

Mammogram If age>39

Medical Clearance If age>44

EKG If age>44

Contact Us

Please use this form to contact us. We will get back to you as soon as possible.